{"id":19,"date":"2015-12-14T18:54:45","date_gmt":"2015-12-14T18:54:45","guid":{"rendered":"http:\/\/elements.chem.umass.edu\/knappgroup\/?page_id=19"},"modified":"2019-07-27T19:05:11","modified_gmt":"2019-07-27T19:05:11","slug":"research","status":"publish","type":"page","link":"https:\/\/elements.chem.umass.edu\/knappgroup\/research\/","title":{"rendered":"Research"},"content":{"rendered":"<p><strong>Hypoxia<\/strong>:<\/p>\n<p>The response to low [O<sub>2<\/sub>] (hypoxia) is crucial for cellular growth, proliferation, and proliferative disease states. My group focuses on the HIF hydroxylases, FIH and PHD, which are human O2-sensing enzymes that hydroxylate specific protein residues on the HIF transcription factor.<\/p>\n<p>These HIF-hydroxylases are nonheme Fe, aKG-dependent oxygenases, but their unique role leads to interesting mechanisms. In addition to mechanistic studies to identify novel intermediates, we combine spectroscopy, crystallography, and medium-throughput assays to screen for inhibitors.<\/p>\n<p><strong>Surface Enhanced Raman (SERS):<\/strong><\/p>\n<p>Detecting metals, metabolites, and biochemical processes with high sensitivity.\u00a0 We are synthesizing compounds to use as hybrid SERS sensors via conjugation of designed molecules onto nanoparticles.<\/p>\n<p><strong>Fluorescent sensors:<\/strong><\/p>\n<p>Explosives detection is of great interest, as well as being an excellent theme to explore chemical sensing approaches. We are developing fluorescent Zn(salophen) complexes for new explosives-detection strategies. This work also tests Zn(salophen) complexes that show promise for cellular imaging.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Hypoxia: The response to low [O2] (hypoxia) is crucial for cellular growth, proliferation, and proliferative disease states. My group focuses on the HIF hydroxylases, FIH and PHD, which are human O2-sensing enzymes that hydroxylate specific protein residues on the HIF transcription factor. These HIF-hydroxylases are nonheme Fe, aKG-dependent oxygenases, but their unique role leads to [&hellip;]<\/p>\n","protected":false},"author":44,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-19","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/pages\/19","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/users\/44"}],"replies":[{"embeddable":true,"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/comments?post=19"}],"version-history":[{"count":4,"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/pages\/19\/revisions"}],"predecessor-version":[{"id":490,"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/pages\/19\/revisions\/490"}],"wp:attachment":[{"href":"https:\/\/elements.chem.umass.edu\/knappgroup\/wp-json\/wp\/v2\/media?parent=19"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}