Andras Dobo (2000-2002)
Project: Protein ion charge state distributions in ESI MS and their correlations with conformations in solution.
Representative publications:
- A. Mohimen, A. Dobo, J.K. Hoerner, I.A. Kaltashov. A chemometric approach to detection and characterization of multiple protein conformers in solution using electrospray ionization mass spectrometry. Anal. Chem., 2003, 75, 4139-4147
- A. Dobo, I.A. Kaltashov. Detection of multiple protein conformational ensembles in solution via deconvolution of charge state distributions in ESI MS. Anal. Chem., 2001, 73, 4763-4773
click here to see all papers co-authored by Dr. Andras Dobo based on his work at UMass-Amherst
Current position: Sigma-Aldrich-Fluka Europe
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Rinat R. Abzalimov (2004-2008)
Project: Top-down hydrogen/deuterium exchange; mass spectrometry of highly heterogeneous biomolecules
Representative publications:
- G. Wang, R.R. Abzalimov, C. Bobst and I.A. Kaltashov. Conformer-specific characterization of nonnative protein states using hydrogen exchange and top-down mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 2013, 110, 20087-20092
- R.R. Abzalimov and I.A. Kaltashov. Electrospray ionization mass spectrometry of highly heterogeneous protein systems: protein ion charge state assignment via incomplete charge reduction. Anal. Chem., 2010, 82, 7523-7526
- I.A. Kaltashov, R.R. Abzalimov. Do ionic charges in ESI MS provide useful information on macromolecular structure? J. Am. Soc. Mass Spectrom., 2008, 19, 1239-1246
click here to see all papers co-authored by Dr. Rinat Abzalimov based on his work at UMass-Amherst
Current position: Director, Biomolecular Mass Spectrometry Facility and Research Assistant Professor, Advanced Science Research Center’s Structural Biology Initiative
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Cedric Bobst (2008-2012, continued as Research Fellow through 2019)
Project: Higher order structure, dynamics and stability of protein-based therapeutics
- C.E. Bobst, J. Sperry, O.V. Friese, I.A. Kaltashov.* Simultaneous evaluation of a vaccine component microheterogeneity and conformational integrity using native mass spectrometry and limited charge reduction. J. Am. Soc. Mass Spectrom. 2021, 32, 1631-1637
- C.E. Bobst, and I.A. Kaltashov. Enhancing the quality of H/D exchange measurements with mass spectrometry detection in disulfide-rich proteins using electron capture dissociation. Anal. Chem. 2014, 86, 5225-5531
- C.E. Bobst, S. Wang, W.-C. Shen, and I.A. Kaltashov. Mass spectrometry study of a transferrin-based protein drug reveals the key role of protein aggregation for successful oral delivery. Proc. Natl. Acad. Sci. U.S.A. 2012, 109, 13544-13548
- C.E. Bobst, J.J. Thomas, P.A. Salinas, P. Savickas, I.A. Kaltashov. Impact of oxidation on protein therapeutics: Conformational dynamics of intact and oxidized acid-β-glucocerebrosidase at near-physiological pH. Protein Sci. 2010, 19, 2366-2378
- C.E. Bobst, M. Zhang, I.A. Kaltashov. Existence of a non-canonical state of iron-bound transferrin at endosomal pH revealed by hydrogen exchange and mass spectrometry. J. Mol. Biol. 2009, 388, 954-967
- C.E. Bobst, R.R. Abzalimov, D. Houde, M. Kloczewiak, R. Mhatre, S.A. Berkowitz, I.A. Kaltashov. Detection and characterization of altered conformations of protein pharmaceuticals using complementary mass spectrometry-based approaches. Anal. Chem. 2008, 80, 7473-7481
Current position: Associate Director, Mass Spectrometry Core Facility, Institute for Applied Life Sciences, UMass-Amherst
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Gregoire Bonvin (2015-2016)
Project: Serum transferrin and non-ferrous metal ion transfer
Representative publications:
- G. Bonvin, C.E. Bobst and I.A. Kaltashov. Interaction of transferrin with non-cognate metals studied by native electrospray ionization mass spectrometry. Int. J. Mass Spectrom., 2017, 420, 74-82
Current position: Chemistry Specialist, the State of Geneva, Geneva, Switzerland
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Vanda Liadinskaia (2018)
Project: Heparin structure/bioactivity studies
Current position: Senior Researcher at Pentair
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Wenhua Yang (2019-2021)
Project: Molecular basis of allergy and autoimmunity
Representative publications:
- W. Yang, D.G. Ivanov and I.A. Kaltashov. Extending the capabilities of intact-mass analyses to monoclonal immunoglobulins of the E-isotype (IgE). MAbs 2022, 14, 2103906
- W. Yang, Z. Tu, D. McClements and I.A. Kaltashov. A systematic assessment of structural heterogeneity and IgG/IgE-binding of ovalbumin. Food Funct. 2021, 12, 8130-8140
- W. Yang, Z. Tu, Q. Li, I.A. Kaltashov and D.J. McClements. Utilization of sonication-glycation to improve the functional properties of ovalbumin: A high-resolution mass spectrometry study. Food Hydrocolloids 2021, 119, 106822
Current position: Associate Professor and Distinguished Scholar, Zhongkai University of Agriculture and Engineering (China)
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Si-Hung Le (2022)
Project: Molecular mechanisms of heparin-induced thrombocytopenia and vaccine-induced immune thrombotic thrombocytopenia
Representative publications:
- D. G. Ivanov, N. Ivetic, Y. Du, S.N. Nguyen, S.H. Le, D. Favre, I. Nazy, and I.A. Kaltashov. Reverse Engineering of a Pathogenic Antibody Reveals the Molecular Mechanism of Vaccine-Induced Immune Thrombotic Thrombocytopenia. J. Am. Chem. Soc. 2023, 145, 25203- 252132
- S.N. Nguyen, S.H. Le, D.G. Ivanov, N. Ivetic, I. Nazy, I.A. KaltashovA. Structural characterization of a pathogenic antibody underlying vaccine-induced immune thrombotic thrombocytopenia (VITT). Anal. Chem., 2024, in press (a pre-print is available at bioRxiv: 2023.2005.2028.542636)
Current position: Applications Scientist, Thăng Long Instruments (Hanoi, Vietnam)
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Son N. Nguyen (2019-2022)
Project: Molecular mechanisms of heparin-induced thrombocytopenia and vaccine-induced immune thrombotic thrombocytopenia
Representative publications:
- D. G. Ivanov, N. Ivetic, Y. Du, S.N. Nguyen, S.H. Le, D. Favre, I. Nazy, and I.A. Kaltashov. Reverse Engineering of a Pathogenic Antibody Reveals the Molecular Mechanism of Vaccine-Induced Immune Thrombotic Thrombocytopenia. J. Am. Chem. Soc. 2023, 145, 25203- 252132
- S.N. Nguyen, S.H. Le, D.G. Ivanov, N. Ivetic, I. Nazy, I.A. KaltashovA. Structural characterization of a pathogenic antibody underlying vaccine-induced immune thrombotic thrombocytopenia (VITT). Anal. Chem., 2024, in press (a pre-print is available at bioRxiv: 2023.2005.2028.542636)
Current position: Senior Research Scientist, Mass Spectrometry Core Facility, UMass Medical School (Worcester, MA)
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